Raspberry bushy dwarf virus pcr
Has PDF. Publication Type. More Filters. First report of raspberry yellows disease caused by raspberry bushy dwarf virus in Japan. Journal of General Plant Pathology. View 1 excerpt, cites background. Raspberry bushy dwarf virus in Slovenia - geographic distribution, genetic diversity and population structure. Epidemiology of viral diseases in raspberry plantation: identification of nepoviruses in the weed association.
Penetration of pollen tubes with accumulated Raspberry bushy dwarf virus into stigmas is involved in initial infection of maternal tissue and horizontal transmission. Biological, serological and molecular characterisation of Raspberry bushy dwarf virus from grapevine and its detection in the nematode Longidorus juvenilis.
European Journal of Plant Pathology. To determine if RBDV-China was a contaminant during the handling of the plants, or if the source was a seedborne virus, the coat protein gene was sequenced and compared to published sequences of RBDV. RNA was extracted from leaves of R.
Products from four separate PCR reactions were sequenced directly or were cloned into the plasmid vector pCR 2. The predicted amino acid sequences were Previously, a maximum of five amino acid differences had been observed in the coat proteins of different RBDV strains 1.
Martin, Robert R. The primers and probes were designed from conserved fragments of the polymerase region of each virus and were effective for the detection of different isolates tested in this study. The RBDV assay amplified a 94 bp amplicon and was able to detect as few as 30 viral copies. Real-time RT-PCR for detection of Raspberry bushy dwarf virus, Raspberry leaf mottle virus and characterizing synergistic interactions in mixed infections.
Journal of Virological Methods, 1 , Sequence analysis revealed the presence of Raspberry bushy dwarf virus RBDV , a pollen-borne Idaeovirus naturally found in several Rubus spp. The presence of RBDV in asymptomatic plants suggests the virus might not be the sole causal agent of the disorder. Further studies are needed to determine the role of RBDV in the observed symptoms, since virus complexes responsible for increased severity of symptoms have been commonly reported in Rubus spp.
References: 1 P.
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